| 角色 | 演员 |
| Dr.Leonard Leaky Hofstader | 约翰尼·盖尔克奇Johnny Galecki |
| Dr.Sheldon Lee Cooper(86 episodes, 2007-2010) | 吉姆·帕森斯Jim Parsons |
| Penny(86 episodes, 2007-2010) | 卡蕾·措科Kaley Cuoco |
| Howard Joel Wolowitz(86 episodes, 2007-2010) | 西蒙·赫尔伯格Simon Helberg |
| Dr.Amy Farrah Fowler(4 episodes, 2010) | 马伊姆·拜力克Mayim Bialik |
| Dr.Rajesh Ramayan Koothrappali | 昆瑙·内亚Kunal Nayyar |
| Leslie Winkle(8 episodes, 2007-2010) | 莎拉·吉尔伯特Sara Gilbert |
| Kurt(3 episodes, 2007-2009) | Brian Wade |
| Dr. Eric Gablehauser(5 episodes, 2007-2008) | Mark Harelik |
| Mrs. Wolowitz(15 episodes, 2007-2010) | 卡萝尔·安·苏希Carol Ann Susi |
| Himself(2 episodes, 2009-2010) | 威尔·惠顿Wil Wheaton |
| Chen(2 episodes, 2007-2008) | 詹姆斯·洪James Hong |
| Althea(3 episodes, 2007-2010) | Vernee Watson-Johnson |
| Dr. V. M. Koothrappali(4 episodes, 2007-2009) | Brian George |
| Cheryl(2 episodes, 2007-2008) | Erin Allin O'Reilly |
| Mrs. Koothrappali(4 episodes, 2007-2009) | Alice Amter |
| Dr.Stephanie Barnett(3 episodes, 2008) | 萨拉·茹 |
| Dr.Bernadette Rostenkowski Wolowitz | 梅丽莎·劳奇Melissa Rauch |
| Herself(2 episodes, 2009-2010) | 凯缇·萨克霍夫Katee Sackhoff |
| Stuart(7 episodes, 2009-2010) | 凯文·苏斯曼Kevin Sussman |
| Dr.Beverly Hofstadter(2 episodes, 2009) | 克里斯汀·芭伦斯基Christine Baranski |
| Dr.Barry Kripke(4 episodes, 2009) | John Ross Bowie |
| Mary Cooper(3 episodes, 2007-2010) | Laurie Metcalf |
| Club Patron(2 episodes, 2009) | Chris Krauser |
| Captain Sweatpants(3 episodes, 2009-2010) | Ian Scott Rudolph |
| Restaurant Patron(2 episodes, 2010) | Erin Pickett |
| Joyce Kim(2 episodes, 2010) | 松村艾丽 |
| Lonely Larry(2 episodes, 2009-2010) | Owen Thayer |
| Alex(2013) | 玛高·哈什曼Margo Harshman |
| Lucy(2013) | 凯特·米库奇Kate Micucci |
| Spock | ---- |
| Arthur Jeffries | 鲍勃·纽哈特 |
